Brassica Genome Gateway
 

Resource collation from the 13th Crucifer Genetics Workshop 24th - 25th March 2002

Resources available for the Multinational Brassica Genome Project (MBGP)
Collated from discussion at Crucifer Genetics Workshop, March 2002.

Resource Species Country Institution Contact Comments
RFLP markers Various USA Wisconsin Tom Osborn
  • Sequenced set due to be submitted to GenBank
Various UK/Canada   Derek Lydiate
  • probes sequenced but currently owned by two breeding companies. However, relative position in A.thaliana can be specified (Parkin et al., in press)
SSR markers Various UK IACR/JIC Martin Trick
  • public domain set generated in BBSRC funded project (Martin Trick, Keith Edwards)
  • Sequence of clones and primers in BrassicaDB
  • Map data for 70 in B. napus in BrassicaDB
  • SSRs currently being evaluated and mapped in B.oleracea and B. rapa at HRI
Various Canada AAFC Andy Sharpe
  • SSRs from AAFC consortium – Consortium due to be polled first half 2002 whether able to release a subset into public domain
Various   Celera  
  • Celera consortium SSRs – unknown status
BAC libraries B. napus France INRA (Genoplante) Boulos Chaloub
  • from Genoplante consortium (separate unit for BAC libraries)
  • 2x BAC libraries available (link)
B. oleracea UK/USA HRI/TAMU Graham King
  • B. oleracea ‘BoB’ – publicly available from TAMU
  • Being contiged as part of UK physical map programme
B. oleracea UK PBL/JIC Ian Bancroft
  • B. oleracea ‘JBo’ - MTA
  • Being contiged as part of UK physical map programme
B. oleracea USA UC Davis Carlos Quiros
  • 18,000 clones (3x) (Carlos Quiros)
  • var. italica ‘Early Big’
B. oleracea USA U Georgia Andy Paterson
  • T01434 rapid cycling line 73,000 (10x)
  • Same line as TIGR B. oleracea genomic sequencing
B. oleracea USA U Georgia Andy Paterson
  • Badger Inbred 16 18,000 (3x)

Resources available for the Multinational Brassica Genome Project (MBGP)
Collated from discussion at Crucifer Genetics Workshop, March 2002 (cont)

BAC libraries B. rapa UK PBL/JIC Ian Bancroft
  • B. rapa ‘JBr’ - MTA
  • Being contiged as part of UK physical map programme
B. rapa S. Korea Taejon Yong Pyo Lim
  • From ‘Chifu’ type
B. napus UK JIC Ian Bancroft
  • B. napus Tapidor large insert "JBnB"
  • Partially contiged as part of EC – China collaboration
  • B. napus Tapidor binary library "JBnY"
  • Tapidor is a parent in "TN" population
B. napus Canada AAFC Isobel Parkin
  • publicly available
  • From DH parent of DH mapping population
B. napus China    
  • 80,000 clones 70kb inserts
  • not public until about 2003

Resources available for the Multinational Brassica Genome Project (MBGP)
Collated from discussion at Crucifer Genetics Workshop, March 2002 (cont)

BAC filter hybridisation   USA U Georgia Andy Paterson
  • overgo probe sets being used for filter hybridisation
ESTs B. napus France INRA (Genoplante) Michel Rennard
  • (+ GSTs)
  • from Geniplante consortium
  • target of 30,000
  • will be available at least one year after obtained. Thus these will not be available until after April/May 2003.
  • (Arabidopsis ESTs were available 6 months after made)
  B. napus Canada AAFC + Andy Sharpe
(Isobel Parkin)
  • website being set up which is searchable and will allow distribution on request subject to MTA
  • Likely to be available in public domain in about 1 year (April 2003?)
  B. napus Germany GABI Friedt
  • cDNAs but industrial consortium, not in public domain
BAC end sequences B. rapa S. Korea   Yong Pyo Lim
  • From ‘Chifu’ library - BAC end sequences and cDNAs (to be put on arrays)
Genomic sequences B. oleracea USA TIGR Chris Town
  • 300,000 in Genbank
  • aiming for ...

Plant Resources

Provisional list of Brassica genetic stocks available for deposit in Ohio Arabidopsis Stock Center (ARBC) c/o Randy Scholl, subject to further discussion and agreement with ABRC
Rapid cycling base populations   USA Williams Rapid cycling stocks (CrGC)
  • One population for each of seven species
  • must be maintained by cross pollination
Mutant stocks   USA Williams CrGC RCB mutants
  • 50 mutant or cytoplasm stocks, mostly B. rapa (Williams)
  USA Tom Osborn/
Rick Amasino
EMS mutants
  • 50-200 B. oleracea EMS mutants, all SC (Osborn, Amasino)
Mapping populations B. oleracea UK Graham King "AG"
  • A12DHd x GDDH33
  • 120 DH lines, SC or semi-SI, (alboglabora x broc.)
  • mapped DH lines available
  UK Mike Kearsey "AG" substitution lines
  • A12DHd x GDDH33
  • mapped substitution lines available
  UK Graham King

 

  • parent lines of other mapping populations
B. oleracea USA Tom Osborn "TB" population
  • 200 DH lines, SC, RC
  • rapid cycling x broccoli
B. rapa S. Korea Yong Pyo Lim "CK" population
  • 86 DH lines, SI (Chifu x ‘Kenshin’) (Lim)
  • SI but being bulked
  • 250 RI lines (i6-i7) to be released when ready
  • Being anchored with public domain SSRs at HRI
B. rapa USA Osborn "IR" population
  • 200 RI lines, SC
  • (RC x Sarson)

 

Mapping populations (cont.) B. rapa

 

   
  • Parents of other pops
B. napus USA Osborn "MS" pop.
  • 100 DH lines
  • SC
  • ‘Major’ x ‘Stella’
  • (winter x spring)
B. napus Canada Lydiate "SG" pop
  • 300 DH lines
  • (spring x resyn.)
B. napus France Rennard "DY" pop
  • 120 DH
  • winter x spring
B. napus China Meng "TN" pop
  • 200 DH lines
  • (winter x semi winter)
  • oilseed double low x high
B. napus      
  • Parents of other pops

Download resource information as a Microsoft Word 97 file

 
 
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