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Qiu D et al. (2006). A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content. Theor. Appl. Genet. 114: 67-80
One of the important obstacles to oilseed rape production in Asia is the lack of cultivars that are able to thrive in local environmental conditions and under low input farming regimes. The aim of IMSORB was to develop resources and knowledge to facilitate subsequent development of new, specifically adapted cultivars of oilseed rape. The project achieved the following:
- A new and economical assay method (based on allele-specific amplification) was developed for Single Nucleotide Polymorphism (SNP) markers in oilseed rape.
- Oilseed rape genomic Bacterial Artificial Chromosome (BAC) clones were assembled into 1,429 sets of overlapping clones (contigs). These constitute a partial physical genome map and represent ca. 20% of the genome of oilseed rape. They are anchored to the Arabidopsis genome sequence and existing Brassica BAC-based physical genome maps.
- Oilseed rape genomic sequences were obtained from 6,900 termini or sub-clones of BAC clones from the partial physical map.
- The sequences of alleles of both Tapidor and Ningyou 7 varieties of oilseed rape were determined for 607 loci, of which 182 were identified as polymorphic.
- A 381-marker genetic linkage map was constructed. This map includes 92 SNP markers developed from the BAC contigs (providing integration with other Brassica physical maps and the Arabidopsis genome sequence) and 184 communal SSR and RFLP markers (providing integration with other Brassica genetic linkage maps).
- An analysis was performed, using the newly developed map, of Quantitative Trait Loci (QTL) for 12 traits of agronomic importance in both Asia and the EU.
Funded by the INCO 2 programme of EC Framework 5
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Page last updated: 23rd April 2007